Inhibition of Escherichia coli
EOS300158
Description
Escherichia coli was cultivated in LB medium. Growth was monitored via measurements of the optical density at 600 nm (turbidity). The values obtained after 24h were analysed. Growth was normalized with respect to the untreated bacteria (0% inhibition) and the "media only" control (100% growth inhibition). 50% growth inhibition was used as threshold for the identification of active compounds. The screening concentration was 50 µM.
Assay setup
Assay stage
Bioassay
Bioassay type
Bioassay setting
Assay organism
BAO tags
Detection method
Physical detection method
Detection instrument
Target
Activities
Concentration unit
Activity determination method
normalization of raw data with respect to growth inhibition [%]; compounds resulting in growth inhibition >50% are defined as active compounds.